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human foreskin fibroblast cell line hff  (ATCC)


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    ATCC human foreskin fibroblast cell line hff
    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell <t>line</t> <t>HFF-1</t> as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
    Human Foreskin Fibroblast Cell Line Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foreskin+fibroblast+hff+1+cells/HFF-1/pmc13200314-41-0-9
    Average 99 stars, based on 1584 article reviews
    human foreskin fibroblast cell line hff - by Bioz Stars, 2026-10
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    1) Product Images from "Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC"

    Article Title: Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC

    Journal: BMC Cancer

    doi: 10.1186/s12885-026-16172-2

    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
    Figure Legend Snippet: Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Techniques Used: Expressing, Flow Cytometry, Immunostaining, Labeling

    Related Articles

    Cell Culture:

    Article Title: Discovery and structure–activity relationship analysis of 2-Pyridyl Thieno[3,2-d]pyrimidine derivatives as promising therapeutic candidates for the treatment of Buruli ulcer
    Article Snippet: .. Briefly, 100 μL of human foreskin fibroblast HFF-1 cells (ATCC SCRC-1041) were seeded at 1×10 4 cells/well in 96-well cell culture plates (CosStar, Washington, DC, United States of America) and incubated overnight to allow cell adhesion. ..

    Article Title: Specificity and functional humoral immune responses induced by the VBI-1501A eVLP HCMV gB vaccine compared to the gB/MF59 vaccine
    Article Snippet: .. Human retinal pigment epithelial (ARPE-19), Human epithelial kidney (HEK293T), and Human foreskin fibroblast (HFF-1) cells were obtained from ATCC and cultured at 37°C in media detailed in Karthigeyan et al. ..

    Article Title: Luminescent Electro‐Spun Nanofibers Crosslinked with Boronic Esters Exhibiting Controlled Release of Carbon Dots for Detection of Wound pHs and Enhanced Antimicrobial
    Article Snippet: .. Human Foreskin Fibroblast HFF‐1 cells (ATCC, SCRC‐1041) were cultured at 37°C in a humidified atmosphere of 5% CO 2 in air, in standard tissue culture plates containing 5 mL Dulbecco's‐modified eagle medium (DMEM, Multicell, Wisent Inc., Canada) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin. .. Disk‐shaped fibrous mats with and without CNDs (≈35 μg and 5 mm diameter) were sterilized under UV for 10 min, and placed in a 96‐well plate (Thermo Fisher Scientific, USA).

    Article Title: Comparative Transcriptomic Analysis Unveils Divergent Effects of FLASH Versus Conventional Irradiation on Skin Cells
    Article Snippet: .. Human foreskin fibroblast (HFF-1) cells were purchased from American Type Culture Collection (ATCC) and cultured in the completed Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) to facilitate robust cell growth., In addition, 1% penicillin (100 U/mL)-streptomycin (100 μg/mL) were added to the completed liquid medium to avoid bacterial contamination. ..

    Article Title: Efficient CRISPR-based genome editing for inducible degron systems to enable temporal control of protein function in large double-stranded DNA virus genomes
    Article Snippet: .. Human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041) were used for HCMV infection and cultured in a 5% CO2 incubator at 37°C. .. Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, LM001-05) supplemented with 10% fetal bovine serum (FBS; Invivogen, F0900-050) and 100 U/ ml penicillin plus 100 μg/ml streptomycin (Gibco, 15140-122).

    Article Title: Luminescent Electro-Spun Nanofibers Crosslinked with Boronic Esters Exhibiting Controlled Release of Carbon Dots for Detection of Wound pHs and Enhanced Antimicrobial.
    Article Snippet: .. Human Foreskin Fibroblast HFF-1 cells (ATCC, SCRC-1041) were cultured at 37◦C in a humidified atmosphere of 5% CO2 in air, in standard tissue culture plates containing 5 mL Dulbecco’smodified eagle medium (DMEM,Multicell, Wisent Inc., Canada) supplemented with 10% fetal bovine serum and 1% penicillin– streptomycin. .. Disk-shaped fibrous mats with and without CNDs (≈35 μg and 5 mm diameter) were sterilized under UV for 10 min, and placed in a 96-well plate (Thermo Fisher Scientific, USA).

    Article Title: Specificity and functional humoral immune responses induced by the VBI-1501A eVLP HCMV gB vaccine compared to the gB/MF59 vaccine.
    Article Snippet: .. Human retinal pigment epithelial (ARPE-19), Human epithelial kidney (HEK293T), and Human foreskin fibroblast (HFF-1) cells were obtained from ATCC and cultured at 37°C in media detailed in Karthigeyan et al.19 .. The HCMV strains used in these assays are derived from bacterial artificial chromosomes (BACs) gifted from Tom Shenk (AD169) or generated in house (Merlin).18 Some Merlin variants expressed GFP from a P2A-GFP cassette after UL36.20 Virus production is further detailed in Karthigeyan et al.19 AD169 revertant virus (AD169r-BAC-GFP) was produced by transfection of HFF-1 cells using Lipofectamine3000 (ThermoFisher Scientific), Merlin BACs were transfected into HFFF-Terts by Amaxa Nucleofector.

    Incubation:

    Article Title: Discovery and structure–activity relationship analysis of 2-Pyridyl Thieno[3,2-d]pyrimidine derivatives as promising therapeutic candidates for the treatment of Buruli ulcer
    Article Snippet: .. Briefly, 100 μL of human foreskin fibroblast HFF-1 cells (ATCC SCRC-1041) were seeded at 1×10 4 cells/well in 96-well cell culture plates (CosStar, Washington, DC, United States of America) and incubated overnight to allow cell adhesion. ..

    Construct:

    Article Title: Efficient CRISPR-based genome editing for inducible degron systems to enable temporal control of protein function in large double-stranded DNA virus genomes
    Article Snippet: The OsTIR1(F74G) coding sequence was amplified by PCR and subcloned into the pDONR221 entry vector (Invitrogen, #12536017). .. The resulting construct was used to transduce human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041). .. The resulting construct was used to transduce human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041).

    Transduction:

    Article Title: Efficient CRISPR-based genome editing for inducible degron systems to enable temporal control of protein function in large double-stranded DNA virus genomes
    Article Snippet: The OsTIR1(F74G) coding sequence was amplified by PCR and subcloned into the pDONR221 entry vector (Invitrogen, #12536017). .. The resulting construct was used to transduce human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041). .. The resulting construct was used to transduce human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041).

    Modification:

    Article Title: Comparative Transcriptomic Analysis Unveils Divergent Effects of FLASH Versus Conventional Irradiation on Skin Cells
    Article Snippet: .. Human foreskin fibroblast (HFF-1) cells were purchased from American Type Culture Collection (ATCC) and cultured in the completed Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) to facilitate robust cell growth., In addition, 1% penicillin (100 U/mL)-streptomycin (100 μg/mL) were added to the completed liquid medium to avoid bacterial contamination. ..

    Infection:

    Article Title: Efficient CRISPR-based genome editing for inducible degron systems to enable temporal control of protein function in large double-stranded DNA virus genomes
    Article Snippet: .. Human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041) were used for HCMV infection and cultured in a 5% CO2 incubator at 37°C. .. Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, LM001-05) supplemented with 10% fetal bovine serum (FBS; Invivogen, F0900-050) and 100 U/ ml penicillin plus 100 μg/ml streptomycin (Gibco, 15140-122).

    Incubator:

    Article Title: Efficient CRISPR-based genome editing for inducible degron systems to enable temporal control of protein function in large double-stranded DNA virus genomes
    Article Snippet: .. Human foreskin fibroblast HFF-1 cells (ATCC, SCRC-1041) were used for HCMV infection and cultured in a 5% CO2 incubator at 37°C. .. Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, LM001-05) supplemented with 10% fetal bovine serum (FBS; Invivogen, F0900-050) and 100 U/ ml penicillin plus 100 μg/ml streptomycin (Gibco, 15140-122).



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    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell <t>line</t> <t>HFF-1</t> as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
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    Image Search Results


    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Journal: BMC Cancer

    Article Title: Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC

    doi: 10.1186/s12885-026-16172-2

    Figure Lengend Snippet: Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Article Snippet: Human foreskin fibroblast cell line HFF-1 was purchased from ATCC (Manassas, USA; SCRC-1041).

    Techniques: Expressing, Flow Cytometry, Immunostaining, Labeling

    Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

    Journal: Macromolecular Bioscience

    Article Title: Lignin Nanoparticles Containing Cobalt‐Cyanine Complexes: Potential Multifunctional Platforms for Photoacoustic Imaging and Photothermal Treatment of Bacterial Biofilms in Chronic Wounds

    doi: 10.1002/mabi.202500532

    Figure Lengend Snippet: Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

    Article Snippet: Bacterial strains ( Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027) and human fibroblast cell line (ATCC‐SCRC‐1041, HFF‐1) were purchased from the American Type Culture Collection (ATCC LGC Standards, Italy).

    Techniques: Fluorescence